Isolation of Rhizospheric Bacteria from Soil and Their Antifungal Activity Against Helminthosporium oryzae the Causal Agent of Brown Spot of Rice

Authors

  • Irfan Ali Department of Plant Pathology, Sindh Agriculture University, Tandojam, Sindh Pakistan
  • Manzoor Ali Abro
  • Rehana Naz Syed Department of Plant Pathology, Sindh Agriculture University Tandojam

DOI:

https://doi.org/10.38211/jms.2023.01.50

Keywords:

Rhizospheric bacteria, antifungal, Helminthosporium oryzae

Abstract

The brown spot of rice caused by Helminthosporium oryzae is a serious disease that affects the yield of rice crops. Brown spots of rice have been reported in almost all rice-growing countries. Therefore, the present study was to explore the management of brown spots of rice. through an antagonistic activity using rhizospheric bacteria. The method employed for soil sample collection was persuaded for bacterial isolation through the serial dilution plate technique. For the results of morphological characteristics of bacterial strain from soils of rice crop collected from Hyderabad. The results of the present study show that many colonies made by bacteria and which were consisted of different sizes, shapes, and colors. Whereas the results of the 15 isolates strains of rhizobacteria, only 06 bacterial strains IAP-1, IAP-2, IAP-04, IAP-09, IAP-12, and IAP-14 were found more effective in antagonizing H.oryzae, under in-vitro conditions. Antagonistic activity of rhizobacteria against H. organize showed significant inhibitory effects. The rhizobacteria showed the primordial result against against H. oryzae and will be helpful as a formulation not only for commercial purposes but also for beneficial biological control against the Brown spot of rice

References

Ahlawat, Y.S. (2007). Crop diseases and their management. Division of Plant Pathology Indian Agricultural Research Institute of New Delhi, 110012.

Akhtar, M. A., Zakria, M., & Abbasi, F. M. (2003). Inoculum build up of bacterial blight of rice in rice-wheat cropping area of Punjab in relation to zero tillage. Asian Journal of Plant Science, 21(3), 548-550.

Akhtar, M. A., Zakria, M., & Abbasi, F.M. (2003). Inoculum buildup of bacterial blight of rice in rice wheat cropping area of Punjab in relation to zero tillage. Asian Journal of Plant Science, 2, 548-550.

Arain, G. N. (2013). Crop manager-agronomy center pivot irrigation system valley irrigation Pakistan (private), Limited. P-1-17.

Arshad, H., Imran, M., Naureen, S., Saleem, K., Ali, S., Jabeen, T. & Babar, M.M. (2015). Morphological and biochemical characterization of Xanthomonas oryzae pv. oryzae isolates collected from Punjab. Advance Journal of Life Science, 72(3), 125-130.

Arumugam, K., Ramalingam, P., & Appu, M. (2013). Isolation of Trichoderma viride and Pseudomonas fluorescens organism from soil and their treatment against rice pathogens. Journal of Microbiology and Biotechnology Research, 34(6), 77-81.

Barnwal, M. K., Kotasthane, A., Magculia, N., Mukherjee, P. K., Savary, S., Sharma, A. K., & Zaidi, N. (2013). A review on crop losses, epidemiology and disease management of rice brown spot to identify research priorities and knowledge gaps. European Journal of Plant Pathology, 36(3), 443-457.

Chaerani, R., & Voorrips, R. E. (2006). Tomato early blight (Alternaria solani): the pathogen, genetics, and breeding for resistance. Journal of general plant pathology, 72, 335-347.

Chakrabarti, N.K. (2001). Epidemiology and disease management of brown sport of rice in India. In: Major fungal disease of Rice: recent Advances. Journal of Crop Research, 80(5), 293-306.

Chaudary, T., Shahid, A. A., Asif, M., Asghar, F., Majeed, R. A., Ali, M., & Anwar, W. (2016). First report of Curvularia lunata causing leaf spot disease of Solanum melongena (Eggplant) in Lahore, Pakistan. Plant Disease, 100(11), 2326-2326.

Datnoff, L.E., Raid, R.N., Snyder, G.H. & & Jones, D.B. (1991). Effect of calcium silicate on blast and Brown spot intensities and yields of rice plant disease. Journal of Agriculture Research and Management, 75(4), 729-735.

Ezaku, A., & Kuka, H. (2006). A historical review of bacterial blight of rice. National Institute geobiology. Journal of Research Bulletin, 80(4), 207-218.

Ginnis, M. C, M.R., Rinalidi M.G.& Winn. R.E. (1986). Emerging agents of phaeohy-phomycosis pathogenic species of Bipolaris and Exserohilum. Journal Clement Microbiology, 24(3), 250-259.

Guri, F., Kapaj, I., Musabelliu, B., Meço, M., Topulli, E., Keco, R., Hodaj, N., Domi, S., Mehmeti, G., Gomez & Paloma, S. (2015). Characteristics of farming systems in Albania. Journal of Technical Research, 60(3), 1172-1183.

Habib, A. M., Richards, P., Cairns, L. S., Rogers, G. J., Bannon, C. A., Parker, H. E., & Gribble, F. M. (2012). Overlap of endocrine hormone expression in the mouse intestine revealed by transcriptional profiling and flow cytometry. Journal of Endocrinology, 53(7), 3054-3065.

Habib, A., Javed, N., Sahi, S. T., & Waheed, M. (2012). Detection of seed borne mycoflora of different coarse and fine rice varieties and their management through seed treatments. Pakistan Journal of Phytopathology, 24(2), 133-136.

Hafeez Abdul. (1995). A guide to plant disease and there control. Food Agriculture Council, Pakistan Ministry of Agriculture Government of Pakistan Karachi. Book P-6.

Haripyaree, A., Gneshwor, K., Damayanti, M., (2010). Electronic Journal of Environmental, Agriculture Food Chemistry, 9(2), 337-344.

Haripyaree, Gneshwor A.K, Damayanti, M. (2010). Efficacy of some fungicides on the development Pyricularia grisea and Heliminthosporium oryzae Electronic. Journal Environment, Agriculture Food Chemistry, 19(2), 337-334.

Ishaq, A., Rehman, A., Zakria, M, & Munir, A (2015). Biochemical characterization of Xanthomonas oryzaepv. Oryzae population from Kallar Belt of Punjab. Pakistan International Journal of Agriculture Innovations and Research, 38(2) 257-266.

Ishiyama, S. (1922). Study on bacterial blight of rice. In Report of Agriculture. Experimental Station, Tokyo, 45, 233-261.

Jabeen, R., Iftikhar, T., & Batool, H. (2012). Isolation, characterization, preservation and pathogenicity test of xanthomonas oryzae. oryzae disease in rice. Pakistan Journal of Botany, 44(1), 261-265.

Jay Parkash, Joshi, M.M., & Mishra, B. (2005). More crop per drop. The Hindu Survey of Indian Agriculture, Ram N. (Ed.), Kasturi and sons Ltd., National press, Chennai, 2, 41146.

Jiskani. (2001). Disease of rice. Industry and economy Pakistan leading business Magazine P-1-4.

Kala, A., Soosairaj, S., Mathiyazhagan, S., & Raja, P. (2015) Isolation and Identification of Xanthomonas oryzae pv. oryzae the causal agent of rice bacterial leaf blight and its activities against of six medicinal plants. Asian Journal of Plant Science and Research, 15(3) (6), 80-83.

Kamal, T., & Mia. M.A.T. (2009). Diversity and pathogen city of the rice brown spot pathogen, Bipolaris Oryzae (Breda de haan) shoem. In Bangladesh assessed by genetic fingerprint analysis. Bangladesh. Journal Botanical, 38(2), 119-125.

Karupasamy, S., Muthuraja, G., & Rajasekaran, K.M., (2009). Advances Journal of Biological Research, 13(4), 196-200.

Lawrence, E., Dantoff, R., & Lentini, S. (1994). Brown Spot in Florida Rice. The Plant Pathology Department, Florida Cooperative extension service, Institute of Food Science and agriculture Science, University of Florida Original publication. 1-3.

Lore, B.P. (2007). Plant Pathology Pathogen and plant Disease book, 122-123.

Malavolta, V.M.A., Parisi, J.J.D., Takada, H.M., & Martins, M.C. (2002). Effect of different incidence levels of Bipolaris oryzae in rice seeds on physiological aspects: seed-seedling transmission and production. Journal of Phytopathology, 28(4), 336-340.

Mia, M. A. T., & Nahar, M. A. (2001). Status of rice seed health in Bangladesh and farmers' seed production and management scenario. Journal of Research Science, 10(1), 81-86.

Mondal, S., Singh, R.P., Mason, E.R., Huerta, E. J., Autrique, E., & Joshi, A.K. (2016). Grain yield, adaptation and progress in breeding for early-maturing and heat-tolerant wheat lines in South Asia. Journal of Field Crops Research, 192, 78-85.

Montesiones, V.V, Ambikapathy & Panneerselvam. A. (1996). Antifungal potentiality of some medicinal plants extracts against Bipolaris oryzae. Asia. Journal Plant Science Research, 1(3), 77-80.

Motaher, M.D., Farjana, S., Abu, H. D., & Asadur, R. (2013). A comparative screening of hybrid, modern varieties and local rice cultivar for brown leaf spot disease susceptibility and yield performance. Journal Archives of Phytopathology and Plant Protection, 3, 01-8.

Motlagh, M. S., & Kaviani, B. (2008). Characterization of new Bipolaris spp.: the causal agent of rice brown spot disease in the north of Iran. International Journal of Agriculture and Biology, 10(6), 638-642.

Naeem, K., Anwar, S. A., Haque, M. I., Riaz, A., & Khan, M. A. (2001). Seed-borne fungi and bacteria of rice and their impact on seed germination. Pakistan Journal of Phytopatholgy, 13(1), 75-81.

New, T.W., Vera, C.M. & Medulla, E.S. (1992). Changes in race frequency of Xathanus Oryzaepv. Oryzae in response to rice cultivators planted in Philippines. Journal of Plant Disease, 76(2), 1029-1032.

Ou, S.H. (1985). Rice Diseases (2nd Ed.) CMI, Kew, England, 370.

Padmanabhan, S.Y. (1973). The great Bengal Famine. Annual. Review of . Phytopathology. 11,11-26.

Pandy. B.P. (2003). Plant Pathology Pathogen and plant disease book P-145-148.

Philip, K., Malek, S. N., Sani, W., Shin, S. K., Kumar, S., Lai, H. S., & Rahman, S. N. (2009). Antimicrobial activity of some medicinal plants from Malaysia. American Journal of Applied Sciences, 6(8), 1613.

Rafi, A., Hameed, A., Akhtar, M. A., Shah, S. M. A., Junaid, M., Shahid, M., & Shah, S. F. (2013). Field based assessment of rice bacterial leaf blight in major rice growing zones of Pakistan. Sarhad Journal of Agriculture, 29(3), 415-422.

Ribot, C., Hirsch, J., Balzergue, S., Tharreau, D., Nottéghem, J. L., Lebrun, M. H., & Morel, J. B. (2008). Susceptibility of rice to the blast fungus, Magnaporthe grisea. Journal of Plant Physiology, 165(1), 114-124.

Roy, M., Shrma, G. D., & Ramana, C. V. (2013). Psudomonads: Potential bio-control agents of rice disease. Research Journal Agriculture and Forestry Science, 70(4), 19-25.

Shoresh, M., Harman, G. E., & Mastouri, F. (2010). Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Journal of Phytopathology, 48(2), 21-43.

Sikia, M., Qing, D. W., Decatur, T., Maurilli, J. Fournier, L., & Tao, P. (2015). A Systematic, Ligation-based approach to stud RAA modifications. Published by Cold Spring Harbor Laboratory Press, 12(1), 2025-2033.

Singh, R. K., Singh, C. V., & Shukla, V. D. (2013). Phosphorus nutrition reduces brown spot incidence in rainfed upland rice. Journal of Rice Research, 30(2), 31-32.

Swings, J., Mooter, M. V. D., Vauterin, L., Hoste, B., Gillis, M., Mew, T. W., & Kersters, K. (1990). Classification of the causal agents of bacterial blight and bacterial leaf streak of rice as Pathovars of Xanthomonas oryzae (ex Ishiyama) sp. International Journal of Systematic & Evolutionary Microbiology, 40(3), 309-311.

Tahvonen, R. (1988). Mycostop-a biological control agent against fungal diseases. Journal of Diseases Research, 49(1), 86-90.

Tewari, S.N., Sukla, H.S., Biswal, M.K., & Nayak, (1988). National Acadmy Science., 40, 369-373.

Vidhyasekaran, K. P. N., Ramanatha, A., Rajappan, K., Paranidharan, V., & Velazhan. (1980). Evaluation of a powder formulation of Pseudomonas fluorescens Pf1 for control of rice sheath blight roots. Journal of Bio-control Science & Technology, 9(1), 67-74.

Webster, R.K., & Gunnell, P.S., (1992). Compendium of rice diseases. St. Paul. American Phytopalogical Society.

Zadok, J. C. (2002). Fifty years of crop protection. (1950-2000). Neth Journal Agriculture Sciences, 181-193.

Downloads

Published

2023-12-25

How to Cite

Ali, I., Abro, M. A., & Syed, R. N. (2023). Isolation of Rhizospheric Bacteria from Soil and Their Antifungal Activity Against Helminthosporium oryzae the Causal Agent of Brown Spot of Rice. Journal of Microbiological Sciences, 2(01), 14–20. https://doi.org/10.38211/jms.2023.01.50