The Prevalence of Carbapenem and Colistin-Resistant Gram-Negative Bacteria Isolated from Hospital-Admitted Patients with Bacteremia

Authors

  • Rao Muhammad Abid KHAN Sindh Institute of Urology and Transplantation (SIUT) https://orcid.org/0000-0002-0239-8927
  • Ali Nadeem Department of Microbiology, Sindh Institute of Urology and Transplantation (SIUT) Karachi-74200, https://orcid.org/0000-0001-7076-3175
  • Muhammad Shaheen Iqbal Department of Bioscience, COMSATS University Islamabad,
  • Faiz Muhammad Department of Microbiology, Balochistan University of Information Technology engineering and management sciences (BUITEMS)
  • Tabbassum Kiran Department of Microbiology, Federal Urdu University of Arts Science and Technology
  • Aamir Ali Department of Microbiology, Shah Abdul Latif University, Khairpur
  • Muneeba Musharraf Department of Microbiology, Sindh Institute of Urology and Transplantation (SIUT) Karachi-74200,
  • Sunil Kumar Dodani Department of Infectious Diseases, Sindh Institute of Urology and Transplantation (SIUT) Karachi-74200,

DOI:

https://doi.org/10.38211/jms.2024.03.69

Keywords:

Blood cultures, Broth microdilution method, Carbapenem resistance, Colistin resistance, Gram-negative bacteria

Abstract

Carbapenem-resistance, a global health concern augmented with resistance against last-resort antibiotic colistin has become a great challenge. Bacteremia caused by Carbapenem-resistant (CR) Gram-negative bacteria (GNB) has been linked to prolonged hospitalization and high mortality. Colistin-resistant Acinetobacter baumannii (CoRAB) represent a serious threat to patients admitted to Intensive Care Unit (ICU) with mortality estimates up to 100%. This study aimed to estimate the current burden of CR and CoR GNB in admitted patients. In this prospective study, a total of 21,600 blood cultures were processed in automated BACTEC system. Conventional methods and Automated Profile Index (API) 20E and 20NE were used for GNB identification. Carbapenem and colistin Antimicrobial Susceptibility Testing (AST) was determined using disc-diffusion and broth microdilution (BMD) methods respectively. The pooled CR was 25.59%. CR was highest in Klebsiella spp. (44.2%) and Acinetobacter baumannii (34.66%). The overall CoR among 2903 GNB was 0.96% while among 743 CR-GNB was 3.76%. CoR rates are lower in Sindh compared to Punjab. CoR has reached up to 5.20% in Klebsiella spp. and up to 3.8% in A. baumannii. All Pseudomonas aeruginosa isolates were sensitive to colistin. Significant proportion of CoRKP and CoRAB in ICU alarms the situation and calls for to seek ways to minimize the emergence of CoR. Klebsiella spp. and A. baumannii remain the predominant CR and CoR GNB in Bloodstream infections (BSI). Presence of CoR-E. coli in pediatric wards highlight the poor hygienic practices and fecal transmission.

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Published

2024-12-30

How to Cite

KHAN, R. M. A., Ali Nadeem, Muhammad Shaheen Iqbal, Faiz Muhammad, Kiran, T., Ali, A., … Sunil Kumar Dodani. (2024). The Prevalence of Carbapenem and Colistin-Resistant Gram-Negative Bacteria Isolated from Hospital-Admitted Patients with Bacteremia. Journal of Microbiological Sciences, 3(01), 30–35. https://doi.org/10.38211/jms.2024.03.69

Issue

Vol. 3 No. 01 (2024): Journal of Microbiological Sciences

Section

Research Articles

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Copyright (c) 2024 Rao Muhammad Abid KHAN, Ali Nadeem, Muhammad Shaheen Iqbal, Faiz Muhammad, Tabbassum Kiran, Aamir Ali, Muneeba Musharraf, Sunil Kumar Dodani

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This work is licensed under a Creative Commons Attribution 4.0 International License.